Automatic Sampling

Some Reactions are Difficult to Sample

Without Affecting Reaction Progression

Reliably taking representative samples of oxygen or moisture-sensitive reactions and reactions at elevated pressure or sub-ambient temperatures is virtually impossible.  Highly toxic reactions present additional challenges.  The unique patented EasySampler probe enables the capture and immediate quenching of reactions to provide a sample representative of a reaction at the time of sampling.

Sample and Quench at Reaction Conditions

How Does it Work?

EasySampler is immersed into a reaction, where it remains for the duration of the experiment. The sample pocket moves out and is filled with a sample. As the pocket withdraws, the sample is quenched immediately, at reaction conditions. The sample is then diluted to a user-specified concentration and ready for offline analysis.

Representative & Reproducible Samples

From Heterogeneous & Multiphase Reactions

Sampling reactions with precision is challenging.  Sampling is especially difficult for heterogeneous and multiphase reactions.  EasySampler samples from the same point in the reactor, into a pocket of fixed volume and includes solids.  Solids dissolution begins immediately, with the quench and dilution steps, delivering dissolved samples to gain representative, reproducible and accurate analytical data.

Automated and Unattended 24/7

Never Miss a Sample Again

Sampling toxic or long reactions, or reactions for Design of Experiments (DoE) studies, can be impractical and cumbersome, leading to blind spots in data.  This often requires repeat experiments.  EasySampler samples the same way every time and can be programmed for continuous sampling operations 24/7 to provide data sets for complete reaction understanding, improving productivity.

Sample Preparation

For HPLC-Ready Concentrations

After quenching, the liquid handling system dilutes the sample to a user-specified concentration and transfers the sample to a vial, making it ready for offline analysis.  These steps can be used for derivatization, decreasing sample preparation time and eliminating human error.

Eliminate User Safety Concerns

Related to Toxic and Hazardous Chemistries

EasySampler fully automates the sampling process, eliminating manual handling of liquids.  This reduces risks and improves operator safety, especially with:

• Highly Toxic Chemistry
• Dangerous Reactions at Elevated Temperatures
• Other Hazardous Reaction Conditions

Faster & Improved Chemical Development

With Integrated Reactor Systems & EasySampler

Common EasySampler applications include:

EasySampler in Recent Journal Publications

Continued automated sampling with the EasySampler supports reaction and impurity profiling studies. A list of publications from peer-reviewed journals focuses on exciting and novel applications of EasySampler by researchers in both academia and industry to support data-rich experimentation to advance their research.

Featured EasySampler Citations

Wang, K., Han, L., Mustakis, J., Li, B., Magano, J., Damon,D., Dion, A., Maloney, M.T., Post, R.J., Li, R., "Kinetic and Data Driven Reaction Analysis for Pharmaceutical Process Development", Ind. Eng. Chem. Res. (2019), Advance article (DOI: 10.1021/acs.iecr.9b03578)

Beutner, G.L., Coombs, J.R., Green, R.A., Inankur, B., Lin, D., Qiu, J., Roberts, F., Simmons, E.M., Wisniewski, S.R., "Palladium-Catalyzed Amidation and Amination of (Hetero)aryl Chlorides under Homogeneous Conditions Enabled by a Soluble DBU/NaTFA Dual-Base System", Org. Process Res. Dev. (2019), 23, 1529-1537.

Mennen, S.M., Alhambra, C., Allen, C.L., Barberis, M., Berritt, S., Brandt, T.A., Campbell, A.D., Castañón, J., Cherney, A.H., Christensen, M., Damon, D.B., de Diego, J.E., García-Cerrada, S., García-Losada,P., Haro, R., Janey, J., Leitch, D.C., Li, L., Liu, F.,  Lobben, P.C., MacMillan, D.W.C., Magano, J., McInturff, E., Monfette, S., Post, R.J., Schultz, D., Sitter, B.J., Stevens, J.M., Strambeanu, I.I., Twilton, J., Wang, K., Zajac, M.A., "The Evolution of High-Throughput Experimentation in Pharmaceutical Development and Perspectives on the Future", Org. Process Res. Dev. (2019) 23, 1213−1242.

Carter, H.L.,  Connor, A.W., Hart, R., McCabe, J., McIntyre, A.C., McMillan, A.E., Monks, N.R., Mullen, A.K., Ronson, T.O., Steven, A., Tomasia, S., Yates, S.D., "Rapid route design of AZD7594", React. Chem. Eng. (2019), Advance Article.

Zawatzky, K., Grosser, S., Welch, C.J., "Facile Kinetic Profiling of Chemical Reactions Using MISER Chromatographic Analysis", Tetrahedron, (2017) 73, 5048-5053.

Gurung, S.R., Mitchell, C., Huang, J., Jonas, M., Strawser, J.D., Daia, E., Hardy, A., O’Brien, E., Hicks, F., Papageorgiou, C.D., "Development and Scale-up of an Efficient Miyaura Borylation Process Using Tetrahydroxydiboron", Org. Process Res. Dev. (2017) 21, 65-74.

Rougeot, C., Situ, H., Cao, B.H., Vlachos, V., Hein, J.E., "Automated Reaction Progress Monitoring of Heterogeneous Reactions: Crystallization-induced Stereoselectivity in amine-catalyzed aldol reactions", React. Chem. Eng. (2017), 2, 226-231.

Malig, T.C., Koenig, J.D.B., Situ, H., Chehal, N.K., Hultinb, P.G., Hein, J.E., ”Real-time HPLC-MS reaction progress monitoring using an automated analytical platform", React. Chem. Eng. (2017) 2, 309-314.

Duan, S., Place, D., Perfect, H.H., Ide, N.D., Maloney, M., Sutherland, K., Price Wiglesworth, K.E.; Wang, K., Olivier, M., Kong, F., Leeman, K., Blunt, J., Draper, J., McAuliffe, M., O’Sullivan, M., Lynch, D. , Palbociclib Commercial Manufacturing Process Development. Part I: Control of Regioselectivity in a Grignard-Mediated SNAr Coupling", Org. Process Res. Dev. (2016), 20, 1191−1202.

Pfizer Evaluates Automated Sampling

Pfizer eliminated sampling challenges with an automated and robust inline method of taking representative samples from reactions, even under difficult conditions, for improved impurity profiling.