Rainin E4 XLS, XLS+ - Instructions - Manual Mode - METTLER TOLEDO
Video
Operating Instructions
User Manual

Rainin E4 XLS, XLS+ - Instructions - Manual Mode

Video
Operating Instructions
User Manual

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Video Transcript:

MANUAL MODE

Sections in this video:

  1. Basic Manual Pipetting
  2. Aspirate or Dispense in Micro Steps
  3. Measure a Volume
  4. Set and Use Fixed Volumes
  5. Use the Cycle Counter

BASIC MANUAL PIPETTING

Move the joystick right or left to navigate to Manual, and push the joystick up to choose it.

Push the joystick up to aspirate. 

Manual mode makes it possible to continuously and incrementally actuate volume control. Nudge the joystick down to partially dispense, then nudge it up to aspirate. Push downward and hold -- to completely dispense.

Joystick right to go into settings. The volume box is lit. Press the joystick in to make changes. Left and right adjust by ten microliters. Up and down are two-tenths. Press the joystick in -- to set.

Move down to Speed -- and press. 

Both aspirate and dispense use the same speed in Manual mode. Left and right decrease or increase it by five, and up and down by one. 

Press DONE to set, and press DONE again to run the volume.

Push and hold the joystick up to fully aspirate. Hold down to dispense completely.

 

ASPIRATE OR DISPENSE IN MICRO STEPS

Aspirate a volume -- and stop. Note "Step" on the button on the right. With the arrow pointed up, push that button to raise the volume in steps of two-tenths. Flick the joystick down a quick stroke to switch the button so it LOWERS the volume in steps of two-tenths. 

Aspiration is responsive from any starting point. Joystick downward and hold to dispense. Press RESET on the left to begin a new cycle.

 

HOW TO MEASURE A MICROVOLUME WITH A PIPETTE

Take a practiced look at the sample in the container, and slightly over-estimate its size. If this is your first time estimating a volume inside this type of container, take a moment to familiarize yourself with how different volumes look -- by dispensing a few volumes into it and noting the levels. 

Push right to go into Settings. Joystick up to Volume, and press in. Set the Volume to your slightly-high estimate. 

Adjust Speed, and press DONE to exit through and run the volume. 

Push the joystick up and hold to aspirate. If you estimated correctly, aspiration should include a small portion of air at the tip. 

Press the button on the right to step down in two-tenth increments. Watch the tip closely, and continue stepping down until the air is gone, with liquid poised to exit the pipette tip opening. Note the volume of your sample. This is your volume.

 

Joystick down to clear the pipette. When the tip is empty, press RESET.

 

SET AND USE FIXED VOLUMES 

 

To dispense fixed volumes that you can save and return to, press the button on the right: OPTIONS.

Press the joystick in to turn Fixed Volume ON – green. Then push DONE on the left. 

Joystick right to go into settings, then push up to the "Fixed Volume 1" box and press the joystick in to open a list of Fixed Volumes.

Joystick to the last fixed volume you want to set, then press the right-side button: END VOLUME.

Joystick to a volume and press the joystick in to highlight. Then joystick left or right, up or down to adjust the volume.

Press DONE to set that volume and move to another.

When each volume is set, press DONE again to exit the Fixed Volumes list.

On the settings screen you can adjust cycle speeds, or joystick Left to exit settings and run the first volume.

Joystick up to aspirate, and down to dispense. 

When complete, joystick right. With the "Fixed Volume 1" box lit, press the joystick in.

Joystick to your next volume and press DONE to exit. Press DONE again if aspirate and dispense speeds are already set.

Joystick up to aspirate, and down to dispense. 

When complete, joystick right. With the "Fixed Volume" box lit, press the joystick in. 

Joystick to your next volume and press DONE to exit. Press DONE again if aspirate and dispense speeds are already set.

Joystick up to aspirate, and down to dispense. 

Press OPTIONS, and press the joystick in to turn off Fixed Volume.

 

USE THE CYCLE COUNTER 

Use the Cycle Counter to let the pipette record progress during an experiment.

In OPTIONS, joystick down to the 2nd screen of options – to Cycle Counter. Press the joystick in to turn Cycle Counter on.

Press DONE to exit, and joystick right to go into settings. Adjust volume and speed settings as you wish. 

Joystick down to the Cycle Count box and press the joystick in to adjust the setting. You can use the joystick to change the number, or press RESET to set the cycle count number to zero.

Aspirate and dispense, and notice the cycle counter registering each cycle.

 

More about E4 XLS+ Electronic Pipettes

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