Titration for Nicotine Content in E-Liquids

Determination of the Nicotine Content in E-Liquids

A non-aqueous acid-base titration with potentiometric indication

The application describes a method to determine the nicotine content in E-liquids by a non-aqueous acid-base titration with potentiometric indication.


Electronic cigarettes are handheld devices that simulate the feeling of smoking. The so-called E-liquid is vaporized by an electronically heated coil and the vapors are inhaled by the user. The liquid usually consists of propylene glycol, glycerol, flavors and nicotine.

Nicotine is a basic alkaloid that is naturally present in the tobacco plant. It is a psychoactive substance with both stimulant and relaxant effects and therefore an important component of E-liquids. Commercially available E-liquids have nicotine concentrations from 3 to 18 mg/mL.


HClO4 + CH3COOH ⇌ CH3COOH2+ + ClO4- (titrant)

CH3COOH2+ + nicotine ⇌ CH3COOH + nicotineH+

This method is developed using a Titrator Excellence system with combined glass pH electrode.

The Application Includes:

  • Introduction
  • Sample Preparation and Procedures
  • Chemistry
  • Instruments and Accessories
  • Method in Detail
  • Results and Remarks

And method files ( .lmt and .xml) for direct import and implementation on the instrument.

Nicotine Content in E-Liquids by Potentiometric Titration
Nicotine Content in E-Liquids by Potentiometric Titration
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