This application describes the ketone body ester determination in sports drinks. The ester is saponified with sodium hydroxide, and the resulting solution is back-titrated with hydrochloric acid.
What Are Ketone Body Esters?
In the human body, ketone bodies serve as energy-providing molecules during fasting or low intake of carbohydrates. The ketone body comprises acetone, acetoacetate, and beta-hydroxybutyrate (BHB). They are used as food supplements, either as a salt or bound to alcohol as an ester.
What Is the Role of Ketone Esters?
During periods of deficient carbohydrate intake, the liver produces ketone esters for its cells to continue energizing the body system. This form of metabolism has caught the interest of athletes and sports organizations to choose ketone bodies as body fuel since they have a significant impact on overall productivity, focus, and mental clarity.
How to Determine the Content of Ketone Esters?
The ester is saponified with sodium hydroxide. The resulting solution is back-titrated with hydrochloric acid to determine the amount of hydroxide used in the saponification reaction.
This Application Note Includes
- An introduction to the method
- Sample preparation and procedures
- Solutions and chemicals
- Chemistry
- Instruments and accessories
- Results and remarks
Method files (.lmt and .xml) are also included for direct import and implementation of the instrument.
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