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Learn how to easily perform the different measurement methods of UV/VIS Excellence Spectrophotometers from METTLER TOLEDO, such as:
- Color measurement
- Fixed wavelength measurements
- Quantification
- Water Testing
- Scanning
- Bio Applications
Learn also how to download and install more than 80 ready-to-use methods from our
How to Perform Color Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to determine the color of a sample with our UV/VIS Excellence spectrophotometers.
We will demonstrate this analysis with the color determination of an electrolyte. As decomposition progresses, the color of the electrolyte turns yellowish. Therefore, we will measure the yellowness index of the electrolyte. This parameter can be used for quality control purposes, as the degree of decomposition reflects the water content within the sample.
How to Perform Fixed Wavelength Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to set up a method for measuring the absorbance at several wavelengths and how to calculate multiple results.
For example, we have chosen the analysis of olive oil samples from our application library.
How to Perform Quantification with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to set up a quantification method with your own calibration curve to quantify an analyte according to the Beer-Lambert law.
The analysis is demonstrated with the quantification of Per- and Polyfluoroalkyl substances, called PFAS for short.
How to Perform Scan Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to measure a full spectrum with our array spectrophotometers.
A full spectrum is very useful to identify the wavelength of a peak maximum to record a calibration curve. For this purpose, we will measure one standard solution of Per- and Polyfluoroalkyl substances, abbreviated PFAS.
How to Perform Color Testing According to Pharmacopeia with UV/VIS Excellence Spectrophotometers
In this video, we will demonstrate how you can easily and correctly determine color according to pharmacopeia with METTLER TOLEDO’s UV7 Excellence spectrophotometers and LabX™ UV/VIS software.
In our Pharma Color Guide, we explain the theory of color determination in more detail.
Mm-hmm. Dobrodošli v našem aplikacijskem laboratoriju Mettler Toledo tukaj v Švici. V tem videu vam bom pokazal, kako lahko enostavno in pravilno določite barvo v skladu s farmakopejo z odličnimi spektrofotometri UV7 podjetja Mettler Toledo in programsko opremo LabX UV-VIS. Kot današnji primer vas bom popeljal skozi postopni vodič za določanje barve v skladu z Evropsko farmakopejo. V našem Pharma Color Guide podrobneje razlagam teorijo določanja barve. Z prenosom vodiča boste prejeli tudi metode LabX, pripravljene za uporabo, za merjenje barve v skladu z obema farmakopejama. Vodič lahko prenesete tukaj. Zato pojdimo zdaj v laboratorij in nastavimo naš instrument. Najprej moramo uvoziti metode v programsko opremo LabX UV-VIS. V ta namen izberemo Import LabX Method in označimo želene metode iz naše ciljne mape. Po uspešnem uvozu so izbrane metode prikazane tukaj v zavihku analize. Ker barvna lestvica Evropske farmakopeje temelji na primerjavi izmerjenih vrednosti CIE Lab z vrednostmi CIE Lab iz barvnih standardov, moramo najprej uvoziti vse referenčne vrednosti v bazo podatkov LabX. Za to moramo izvesti ustrezno metodo Caleb table. V tem primeru bom primerjal samo s standardno rjavo P_. Izberemo metodo EOP Caleb table B_ in zaženemo nalogo na priključenem spektrofotometru UV7. V urejevalniku naloge naložimo datoteko s parametri naloge za rjavo barvo. Datoteka s parametri naloge je del paketa metode in vsebuje referenčne vrednosti za vse standarde rjave barve. S klikom na OK se metoda zažene na instrumentu, referenčne vrednosti pa se shranijo v bazo podatkov Levix. Ti začetni koraki je treba izvesti le enkrat. Vrednosti bodo zdaj ostale v bazi podatkov. Nastavitev je zdaj končana in pripravljeni smo meriti vzorce v skladu z Evropsko farmakopejo. Za začetek analize vzorcev zaženemo metodo UP 00B in določimo seznam vzorcev. Po potrditvi se metoda zažene na instrumentu. Najprej moramo izmeriti slepi vzorec. Za to uporabljam destilirano vodo. Po določitvi slepega vzorca je operater pozvan, naj vstavi prvi vzorec. Takoj ko je meritev končana, je kot rezultat prikazan barvni standard, ki je najbližje vrednosti vzorca. Upam, da vam je ta kratek video pomagal razumeti, kako določiti barvo v skladu z Evropsko farmakopejo. Postopek za ameriško farmakopejo je zelo podoben, naš Pharma Color Guide pa vsebuje podroben opis skupaj z nekaj osnovne teorije. Hvala, ker ste si ogledali ta video, in ne spreglejte naših drugih videoposnetkov, ki pojasnjujejo različne merilne kategorije in kako prenesti več metod, pripravljenih za uporabo, iz naše aplikacijske knjižnice na mt.com.
How to Perform Bio Applications with UV/VIS Excellence Spectrophotometers
In this video, we will demonstrate how you can perform bio applications as DNA analysis and proten measurements with METTLER TOLEDO’s UV5Nano and UV5Bio Excellence spectrophotometers.
In our Life Science Toolbox you will find helpful theory and and best practices to improve your results.
Method Retrieval and Installation
How to Receive and Install Methods on UV/VIS Excellence Spectrophotometers
Did you know that there are more than 80 ready-to-use application methods available for UV/VIS Excellence spectrophotometers? In this video, you will learn where to find the application packages and how to download them from mt.com as well as how to install a new method on your instrument.
