This page describes the volumetric Karl Fischer technique for water content in protein powders using the EVA titrator, highlighting its unique drift stability and compact design.
By downloading this application package, you will receive a PDF of the application note as well as the required data for the LabX™ titration software method.
The volumetric Karl Fischer titration method measures the water content in various samples. Equal volumes of water in the sample react with equal volumes of iodine in the titrant. When all water molecules are consumed, the excess iodine present will no longer be reduced; thus, the endpoint is determined.
Why measure water in protein powders?
Protein powders- whether derived from milk, eggs, or plants- are a staple in nutrition and wellness. Yet, their quality and shelf life hinge on one critical factor: water content. Too much water can trigger microbial growth and chemical instability, while too little can compromise solubility and usability. Ensuring the proper moisture balance is essential for manufacturers and labs striving to deliver safe, effective products.
What makes the EVA tirator special?
The EVA Volumetric Karl Fischer titrator brings a new confidence level to water content determination in protein powders. The EVA system is designed for routine laboratory needs and introduces a novel drift stability feature. This innovation allows titrations to start and stop based on stable drift conditions, rather than relying solely on preset drift values. The results are repeatable and accurate, even when analyzing challenging samples or working in environments with variable baseline drift.
How does the method work?
The workflow is straightforward and efficient. Prepare the titration cell with a methanol-formamide solvent mixture and a buffering agent. Once the system confirms a stable drift, directly add your protein powder sample (whey, casein, soy, egg white, brown rice, or pea protein) to the vessel. The EVA titrator’s intuitive interface guides you through each step, from conditioning the system to capturing precise results.
