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Learn how to easily perform the different measurement methods of UV/VIS Excellence Spectrophotometers from METTLER TOLEDO, such as:
- Color measurement
- Fixed wavelength measurements
- Quantification
- Water Testing
- Scanning
- Bio Applications
Learn also how to download and install more than 80 ready-to-use methods from our
How to Perform Color Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to determine the color of a sample with our UV/VIS Excellence spectrophotometers.
We will demonstrate this analysis with the color determination of an electrolyte. As decomposition progresses, the color of the electrolyte turns yellowish. Therefore, we will measure the yellowness index of the electrolyte. This parameter can be used for quality control purposes, as the degree of decomposition reflects the water content within the sample.
How to Perform Fixed Wavelength Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to set up a method for measuring the absorbance at several wavelengths and how to calculate multiple results.
For example, we have chosen the analysis of olive oil samples from our application library.
How to Perform Quantification with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to set up a quantification method with your own calibration curve to quantify an analyte according to the Beer-Lambert law.
The analysis is demonstrated with the quantification of Per- and Polyfluoroalkyl substances, called PFAS for short.
How to Perform Scan Measurements with UV/VIS Excellence Spectrophotometers
In this video, you will learn how to measure a full spectrum with our array spectrophotometers.
A full spectrum is very useful to identify the wavelength of a peak maximum to record a calibration curve. For this purpose, we will measure one standard solution of Per- and Polyfluoroalkyl substances, abbreviated PFAS.
How to Perform Color Testing According to Pharmacopeia with UV/VIS Excellence Spectrophotometers
In this video, we will demonstrate how you can easily and correctly determine color according to pharmacopeia with METTLER TOLEDO’s UV7 Excellence spectrophotometers and LabX™ UV/VIS software.
In our Pharma Color Guide, we explain the theory of color determination in more detail.
嗯嗯。 歡迎來到我們位於瑞士的 Mettler Toledo 應用實驗室。在這段影片中，我將示範如何使用 Mettler Toledo 的 UV7 excellent 分光光度計與 LabX UV-VIS 軟體，輕鬆且正確地依藥典測定顏色。今天的示例中，我將帶您一步一步了解依歐洲藥典進行顏色判定的教學。 在我們的 Pharma Color Guide 中，我會更詳細說明顏色測定的理論。下載本指南後，您還可獲得可直接使用的 LabX 方法，用於依兩部藥典進行顏色測量。您可以在此下載指南。現在讓我們到實驗台上設定儀器。首先，我們需要將方法匯入 LabX UV-VIS 軟體。 為此，我們選擇 Import LabX Method，並在目標資料夾中標記所需的方法。匯入成功後，所選方法會顯示在這裡的分析分頁中。由於歐洲藥典的色階是以量測到的 CIE Lab 值與色標的 CIE Lab 值進行比較為基礎，因此我們首先需要將所有參考值匯入 LabX 資料庫。 為此，我們需要執行相應的 Caleb table 方法。在這個例子中，我只會與標準棕色 P_ 進行比較。我們選擇方法 EOP Caleb table B_，並在連接的 UV7 分光光度計上啟動任務。在任務編輯器中，我們上傳棕色的任務參數檔。 任務參數檔是方法套件的一部分，內含所有棕色色標準的參考值。按一下 OK 後，方法即會在儀器上啟動，參考值也會儲存在 Levix 資料庫中。這些初始步驟只需要執行一次。之後這些數值將保留在資料庫中。現在設定已完成，我們可以依歐洲藥典開始測量樣品了。 若要開始樣品分析，我們啟動方法 UP 00B 並定義樣品清單。確認後，方法便會在儀器上啟動。首先我們需要測量空白，這裡我使用蒸餾水。完成空白測定後，系統會提示操作人員放入第一個樣品。 測量完成後，系統會顯示與樣品值最接近的色標作為結果。希望這段短片能幫助您了解如何依歐洲藥典判定顏色。美國藥典的工作流程非常相似，而我們的 Pharma Color Guide 也提供了詳細說明及部分背景理論。 感謝您觀看這段影片，也別錯過我們其他影片，這些影片會說明各種測量類別，以及如何從 mt.com 的應用程式庫下載更多可直接使用的方法。
How to Perform Bio Applications with UV/VIS Excellence Spectrophotometers
In this video, we will demonstrate how you can perform bio applications as DNA analysis and proten measurements with METTLER TOLEDO’s UV5Nano and UV5Bio Excellence spectrophotometers.
In our Life Science Toolbox you will find helpful theory and and best practices to improve your results.
Method Retrieval and Installation
How to Receive and Install Methods on UV/VIS Excellence Spectrophotometers
Did you know that there are more than 80 ready-to-use application methods available for UV/VIS Excellence spectrophotometers? In this video, you will learn where to find the application packages and how to download them from mt.com as well as how to install a new method on your instrument.
