在线HPLC反应分析
借助DirectInject-LC™，HPLC可近乎实时地理解反应和结晶过程。具有独有专利的全自动快速反应取样工具将HPLC转变为一种强大的在线反应监控新技术。
利用世界领先的、专门用于反应分析和建模的iC软件分析数据，可以更深入地理解反应、加快过程和产品开发。
在线HPLC分析解决方案
轻松原位取样
EasySampler探头和Flow Sampler实现全自动、可重复且具有代表性的反应取样、处理和进样。独有专利EasySampler™技术可连续采集反应条件下的样品，并实时进行稀释、淬灭处理，该技术可用于分析复杂、多相和具有挑战性的化学过程。
- EasyFrit自清洁过滤器确保悬浮体系下只针对溶液相的取样
- Flow Sampler模块可从连续流反应中精确取样
- 间歇和连续过程均适用
iC LC软件
基于iC平台构建，轻松集成PAT控制、分析和正交数据，实现可视化。
可设置自动取样时间间隔，并触发HPLC方法执行。集成色谱数据系统(CDS)可实现实时图片可视化，更便于分析。
可查看三维表面图和二维色谱图，更直观
- 灵活切换HPLC检测器通道
- 双击即可查看峰形（峰高、峰面积等）
- 所有通道的峰可同时显示、叠加
轻松传输和集成数据用于建模，以进一步理解反应和结晶过程。
全面监控和理解反应、结晶过程 — 应用示例
连续手性结晶
研究人员实现了对连续手性结晶的过饱和控制，并且获得高收率的对映纯产物。
提升整体反应效率
通过对整个反应过程的机理理解，反应速率提高了5倍，并减少了样品分解。
结构相似的物质
通过反应理解，完全实现了对多种类似中间体、空气敏感交叉偶联过程中重要有机导体的合成控制。
Reaction Analysis Resources
DirectInject-LC in Peer-Reviewed Publications
Below is a selection of publications featuring online HPLC instruments:
- Ji, Y., Ayesa, U., Lévesque, F., & Qin, Y. (2025). Application of Direct Inject Liquid Chromatography (DILC) as Real-Time Process Analytical Technology for flow reactions. Organic Process Research & Development, 29(11), 2758–2763. https://doi.org/10.1021/acs.oprd.5c00252
- Qin, Y., Ayesa, U., Lévesque, F., & Ji, Y. (2025). Novel Application of Direct Inject Liquid Chromatography (DILC™) as Real-time Process Analytical Technology for Flow Reactions. ChemRxiv. https://doi.org/10.26434/chemrxiv-2025-c3680
- Cai, I., Malig, T. C., Kurita, K. L., Derasp, J. S., Sirois, L. E., & Hein, J. E. (2024). Investigating the Origin of Epimerization Attenuation during Pd-Catalyzed Cross-Coupling Reactions. ACS Catalysis, 14(16), 12331–12341. https://doi.org/10.1021/acscatal.4c03401
- Shi, Y., Derasp, J. S., Guzman, S. M., Patrick, B. O., & Hein, J. E. (2024). Halide salts alleviate TMSOK inhibition in Suzuki–Miyaura Cross-Couplings. ACS Catalysis, 14(16), 12671–12680. https://doi.org/10.1021/acscatal.4c02407
- Kukor, A. J., St-Jean, F., Stumpf, A., Malig, T. C., Piechowicz, K. A., Kurita, K., & Hein, J. E. (2023). Guided optimization of a crystallization-induced diastereomer transformation to access a key navoximod intermediate. Reaction Chemistry & Engineering, 8(6), 1294–1299. https://doi.org/10.1039/d3re00077j
- Deem, M. C., Derasp, J. S., Malig, T. C., Legard, K., Berlinguette, C. P., & Hein, J. E. (2022). Ring walking as a regioselectivity control element in Pd-catalyzed C-N cross-coupling. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30255-1
- Kukor, A. J., Depner, N., Cai, I., Tucker, J. L., Culhane, J. C., & Hein, J. E. (2022). Enantioselective synthesis of (−)-tetrabenazine via continuous crystallization-induced diastereomer transformation. Chemical Science, 13(36), 10765–10772. https://doi.org/10.1039/d2sc01825j
- Malig, T. C., Kumar, A., & Kurita, K. L. (2022). Online and in situ monitoring of the exchange, transmetalation, and Cross-Coupling of a Negishi reaction. Organic Process Research & Development, 26(5), 1514–1519. https://doi.org/10.1021/acs.oprd.2c00081
- Christensen, M., Yunker, L. P. E., Shiri, P., Zepel, T., Prieto, P. L., Grunert, S., Bork, F., & Hein, J. E. (2021). Automation isn’t automatic. Chemical Science, 12(47), 15473–15490. https://doi.org/10.1039/d1sc04588a
- Sato, Y., Liu, J., Kukor, A. J., Culhane, J. C., Tucker, J. L., Kucera, D. J., Cochran, B. M., & Hein, J. E. (2021). Real-Time monitoring of Solid–Liquid slurries: Optimized synthesis of tetrabenazine. The Journal of Organic Chemistry, 86(20), 14069–14078. https://doi.org/10.1021/acs.joc.1c01098
- Kukor, A. J., Guy, M. A., Hawkins, J. M., & Hein, J. E. (2021). A robust new tool for online solution-phase sampling of crystallizations. Reaction Chemistry & Engineering, 6(11), 2042–2049. https://doi.org/10.1039/d1re00284h
- Malig, T. C., Yunker, L. P. E., Steiner, S., & Hein, J. E. (2020). Online HPLC Analysis of Buchwald-Hartwig Aminations from Within an Inert Environment. Analytical Chemistry. https://doi.org/10.26434/chemrxiv.12798083.v1
- Rougeot, C., Situ, H., Cao, B. H., Vlachos, V., & Hein, J. E. (2017). Automated reaction progress monitoring of heterogeneous reactions: crystallization-induced stereoselectivity in amine-catalyzed aldol reactions. Reaction Chemistry & Engineering, 2(2), 226–231. https://doi.org/10.1039/c6re00211k