什麼是 DirectInject-LC？
傳統 HPLC 儀器轉型為在線分析
透過 DirectInject-LC，HPLC 現在可用於近乎即時的反應與結晶過程研究：
全自動化：快速的反應採樣與進樣，將 HPLC 轉變為強大的在線監測工具。
數據整合：配合世界領先的 iC 軟體（專為反應分析與建模設計），能更深入地洞察反應動力學，加速產品與製程開發。
定量分析：獲得反應物濃度、動力學數據、結晶過程以及雜質概況（Impurity Profiles）。
Discover online HPLC analysis solutions.
什麼是 DirectInject-LC？
無需手動的 In-Situ 採樣：
提升高效能液相層析儀的精準度
利用 EasySampler 探頭與 Flow Sampler，實現無需手動操作且具高重複性的反應採樣、製備與進樣：
代表性採樣：在反應條件下立即淬滅，確保樣品能代表反應真實狀態。
處理挑戰性化學：適用於複雜、多相 (Multiphase) 的反應環境。
EasyFrit：配備自清潔過濾器，實現選擇性的液相採樣。
Flow Sampler 模組：專為連續流反應 (Continuous Flow) 設計的精確採樣方案。
DirectInject-LC 在線 HPLC 儀器的綜合反應監測— 應用案例
連續手性結晶的關鍵挑戰
在連續製程中，環境變數（如溫度、流速、濃度）的微小波動都會影響結晶品質。對於手性分子而言，若無法精確控制過飽和度，容易導致不想要的對映異構體析出，或產生多晶型（Polymorphism）轉變。
反應速率與穩定性的權衡
在化學開發中，加速反應（如提高溫度或增加試劑濃度）往往會伴隨副反應增加或產物分解的風險。要在提升 5 倍速率的同時維持穩定性，必須精確掌握反應動力學。
高靈敏度與複雜競爭反應
「自動化反應器」與「即時分析工具」結合時，即使是極其複雜且敏感的有機合成，也能轉化為高度受控且可預測的精密製程。
反應分析相關資源
DirectInject-LC in Peer-Reviewed Publications
Below is a selection of publications featuring online HPLC instruments:
- Ji, Y., Ayesa, U., Lévesque, F., & Qin, Y. (2025). Application of Direct Inject Liquid Chromatography (DILC) as Real-Time Process Analytical Technology for flow reactions. Organic Process Research & Development, 29(11), 2758–2763. https://doi.org/10.1021/acs.oprd.5c00252
- Qin, Y., Ayesa, U., Lévesque, F., & Ji, Y. (2025). Novel Application of Direct Inject Liquid Chromatography (DILC™) as Real-time Process Analytical Technology for Flow Reactions. ChemRxiv. https://doi.org/10.26434/chemrxiv-2025-c3680
- Cai, I., Malig, T. C., Kurita, K. L., Derasp, J. S., Sirois, L. E., & Hein, J. E. (2024). Investigating the Origin of Epimerization Attenuation during Pd-Catalyzed Cross-Coupling Reactions. ACS Catalysis, 14(16), 12331–12341. https://doi.org/10.1021/acscatal.4c03401
- Shi, Y., Derasp, J. S., Guzman, S. M., Patrick, B. O., & Hein, J. E. (2024). Halide salts alleviate TMSOK inhibition in Suzuki–Miyaura Cross-Couplings. ACS Catalysis, 14(16), 12671–12680. https://doi.org/10.1021/acscatal.4c02407
- Kukor, A. J., St-Jean, F., Stumpf, A., Malig, T. C., Piechowicz, K. A., Kurita, K., & Hein, J. E. (2023). Guided optimization of a crystallization-induced diastereomer transformation to access a key navoximod intermediate. Reaction Chemistry & Engineering, 8(6), 1294–1299. https://doi.org/10.1039/d3re00077j
- Deem, M. C., Derasp, J. S., Malig, T. C., Legard, K., Berlinguette, C. P., & Hein, J. E. (2022). Ring walking as a regioselectivity control element in Pd-catalyzed C-N cross-coupling. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30255-1
- Kukor, A. J., Depner, N., Cai, I., Tucker, J. L., Culhane, J. C., & Hein, J. E. (2022). Enantioselective synthesis of (−)-tetrabenazine via continuous crystallization-induced diastereomer transformation. Chemical Science, 13(36), 10765–10772. https://doi.org/10.1039/d2sc01825j
- Malig, T. C., Kumar, A., & Kurita, K. L. (2022). Online and in situ monitoring of the exchange, transmetalation, and Cross-Coupling of a Negishi reaction. Organic Process Research & Development, 26(5), 1514–1519. https://doi.org/10.1021/acs.oprd.2c00081
- Christensen, M., Yunker, L. P. E., Shiri, P., Zepel, T., Prieto, P. L., Grunert, S., Bork, F., & Hein, J. E. (2021). Automation isn’t automatic. Chemical Science, 12(47), 15473–15490. https://doi.org/10.1039/d1sc04588a
- Sato, Y., Liu, J., Kukor, A. J., Culhane, J. C., Tucker, J. L., Kucera, D. J., Cochran, B. M., & Hein, J. E. (2021). Real-Time monitoring of Solid–Liquid slurries: Optimized synthesis of tetrabenazine. The Journal of Organic Chemistry, 86(20), 14069–14078. https://doi.org/10.1021/acs.joc.1c01098
- Kukor, A. J., Guy, M. A., Hawkins, J. M., & Hein, J. E. (2021). A robust new tool for online solution-phase sampling of crystallizations. Reaction Chemistry & Engineering, 6(11), 2042–2049. https://doi.org/10.1039/d1re00284h
- Malig, T. C., Yunker, L. P. E., Steiner, S., & Hein, J. E. (2020). Online HPLC Analysis of Buchwald-Hartwig Aminations from Within an Inert Environment. Analytical Chemistry. https://doi.org/10.26434/chemrxiv.12798083.v1
- Rougeot, C., Situ, H., Cao, B. H., Vlachos, V., & Hein, J. E. (2017). Automated reaction progress monitoring of heterogeneous reactions: crystallization-induced stereoselectivity in amine-catalyzed aldol reactions. Reaction Chemistry & Engineering, 2(2), 226–231. https://doi.org/10.1039/c6re00211k