隨著生物製品市場的不斷發展，人們日益將重心放在改進生物過程以最大限度提高產量和降低成本上。通過過程分析技術(PAT)原位即時地監測關鍵工藝參數對監測、確定、優化和控制生物過程來說是很有必要的。 與離線技術相比，原位技術具有幾大優勢，包括無需採樣和繁瑣的樣品製備，不會破壞工藝過程和過程組分，並且提供關於工藝過程在醱酵罐/生物反應器中的即時資訊。此外，原位技術提供的即時資訊可立刻的檢測到工藝過程的偏差，從而實現工藝控制。
梅特勒-托利多提供的工具和即時資料有助於成功地監測生物過程和所需產品基質的 酶轉化 。上游醱酵和細胞培養應用包括監測整個細胞培養/醱酵過程中的關鍵基質組分、代謝物和產物，以及監測細胞/微生物種群和形態的改變（包括細胞或微生物的聚集或絮凝）。下游處理應用包括細胞碎屑絮凝、蛋白結晶和溶解。
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