UV Vis spectrophotometry

UV Vis Spectrophotometry

Instruments, Accessories, Services, and Software for UV Vis Spectrophotometry

Spectrophotometry is a powerful technique used to measure a sample's light absorption and transmission properties, providing valuable insights into its composition and chemical properties. Whether you're working in life sciences, quality control, environmental science, or any other field where accurate and reliable measurements are critical to success, our spectrophotometric solutions offer performance, ease of use, and scalability, helping you achieve faster, more precise results with every analysis.

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What is UV Vis spectrophotometry?

Spectrophotometry is the measurement of light absorption by a substance as a function of wavelength. It is commonly used in analytical chemistry, biochemistry, and molecular biology.

What are the applications of UV Vis spectrophotometry?

UV Vis spectrophotometry can be used to determine the concentration of a solution, to identify and quantify the components of a mixture, and to study the kinetics of a reaction.

Find our more in the UV Vis Application Library

What is the difference between spectrophotometry and spectroscopy?

Spectroscopy is a broader term that refers to the study of the interaction between electromagnetic radiation and matter. Spectrophotometry is a specific type of spectroscopy that measures the absorption of light by a substance.

Learn more about the theory of UV Vis spectrophotometry in the Easy Spectrophotometry Guide or the Spectrophotometry Applications and Fundamentals Guide

How does UV Vis spectrophotometry work?

A UV Vis spectrophotometer measures the amount of light absorbed by a sample at a specific wavelength. The amount of light absorbed is directly proportional to the concentration of the substance in the sample.

What are the advantages of using UV Vis spectrophotometry?

UV Vis spectrophotometry is a fast, accurate, and non-destructive method of analyzing samples. It can be used for a wide range of substances and is widely accepted as a standard analytical technique in many industries.

How is UV Vis spectrophotometry used in the pharmaceutical industry?

UV Vis spectrophotometry is used in the pharmaceutical industry to analyze the purity, identity, and strength of drugs. It can also be used to monitor the degradation of drugs over time.

To learn more about how spectrophotometry can ensure therapeutic quality, read our customer story

What is a blank in UV Vis spectrophotometry?

A blank is a sample that contains all the reagents except the substance being tested. It is used to calibrate the spectrophotometer and to correct for any background absorption or interference.

Can UV Vis spectrophotometry be used to analyze biological samples?

Yes, UV Vis spectrophotometry can be used to analyze biological samples such as proteins, nucleic acids, and enzymes. It can also be used to quantify the amount of a particular compound in a biological sample.

Learn more in the Life Science Toolbox for UV Vis Spectrophotometry

How can I ensure the accuracy of my spectrophotometry measurements?

To ensure accuracy, it is important to properly calibrate the spectrophotometer, use appropriate reagents and standards, and follow a standardized protocol for sample preparation and measurement. It is also important to validate your results by comparing them to other methods of analysis.

Find out more about the METTLER TOLEDO service offerings for UV Vis calibration