Bioreactor Single-Use Sensors

Analytical Reusable & Single-Use Sensors


Differences in Operation When Used in Single-Use Bioreactors

The deployment of single-use bioreactors (SUBs) is well established in the bioprocessing industry. SUB technology for mammalian cell culture and microbial cells has achieved major breakthroughs in process safety and reliability to comply with the industry’s strict requirements.

This application note explains the differences in operation of pH and DO single-use and reusable sensors when employed in single-use bioreactors.

Regardless of whether reusable or single-use pH and DO sensors are selected for your SUBs, METTLER TOLEDO can offer highly reliable sensors of both types. Single-use InSUS 310 sensors for pH, and InSUS 607 sensors for DO measurement are supplied as integrated components in SUB assemblies from leading single-use stirred-tank bioreactor manufacturers.

The METTLER TOLEDO InSUS sensor series for single-use technology bioprocesses covers:

  • Potentiometric pH sensors with a measurement range from pH 3–10
  • Optical dissolved O2 sensors based on fluorescence quenching measurement principle

The output signals of InSUS sensors are identical to most of the common reusable sensors. Consequently, seamless integration into an existing transmitter or biocontroller process environment is straightforward. Inventory control of bags, both at bag manufacturer and end-user sites, is enhanced thanks to a pH sensor shelf life of up to 30 months. The sensors’ long term measurement stability allows for reliable use, especially in intensified bioprocesses.