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Determination of Vitamin A

Precise measurement in Flour, Tablets and Cod Liver Oil using UV/Vis Spectroscopy

The application is based on the measurement of the absorbance of vitamin A extracted in diethyl ether at a wavelength of 325 nm using METTLER TOLEDO UV/VIS Excellence spectrophotometer.

What is the importance of Vitamin A ?

Vitamin A is essential for the maintenance and normal function of the eyes and epithelial tissues lining the respiratory and reproductive tracts, and bone development in animals and humans. The deficiency of vitamin may decrease the resistance to infections, and cause xeropthalmia. Therefore, the consumption of food products containing vitamin A is necessary.

This application note includes:

  • Introduction
  • Sample Preparation and Procedures
  • Solutions and Chemicals
  • Instruments and Accessories
  • Method in Detail
  • Results and Remarks

And method files (.lmt and .xml) for direct import and implementation on the instrument.

Vitamin A in Flour, Tablets and Cod Liver Oil with UV Vis Spectoscopy
Vitamin A in Flour, Tablets and Cod Liver Oil with UV Vis Spectoscopy