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OD600 of Escherichia Coli

Optical Density of Biological Cultures using UV/VIS spectrophotometer

The application focuses on measuring OD600 of Escherichia coli (E. coli) bacteria using METTLER TOLEDO UV/VIS spectrophotometer.

What is OD600?

The turbidity measurement of microbial cultures is a widely used method to determine the cell number of growing microorganisms by measuring its OD600. OD600 stands for the estimation of optical density of biological cultures at 600 nm.

This application note includes:

  • Introduction
  • Sample Preparation and Procedures
  • Solutions and Chemicals
  • Instruments and Accessories
  • Method in Detail
  • Results and Remarks

And method files (.lmt and .xml) for direct import and implementation on the instrument.

OD600 of Escherichia Coli UV Vis Spectoscopy
OD600 of Escherichia Coli UV Vis Spectoscopy