at the Canadian Centre for DNA Barcoding
Housed in the Biodiversity Institute at the University of Guelph in Toronto, the Canadian Centre for DNA Barcoding – or CCDB – is an integral part of International Barcode of Life Project. The project is creating a barcode reference database of short standardized gene regions. Its mission: identification and discovery of five million specimens from more than 500,000 species in 5 years. Since 2009, the Centre has processed roughly 300,000 samples a year from animal, plant and fungal sources. Once at the CCDB, samples follow a typical genomics workflow with DNA extraction and PCR amplification. Downstream analysis methods include sequencing DNA barcodes for animals that comprise roughly 650 base pairs of COX gene. For plants, rbcL and matK chloroplast genes are used.