Ion Exchange with PureSpeed Tips
Ion exchange chromatography separates molecules by exploiting differences in their overall charge characteristics. Its simplicity makes this type of chromatography a powerful tool for separating proteins, nucleic acids, and other molecules such as peptides. With no special tagging of biomolecules needed, ion exchange is a good general solution for purification. The buffer conditions used in ion exchange are also usually compatible with the molecules of interest, so functional activity is preserved during the purification process.