Application Note: Dye- and Probe-based qPCR - METTLER TOLEDO
White Paper

Application Note: Dye- and Probe-based qPCR

White Paper

Using the 0.5 to 20 μL Liquidator™ 96

Quantitative PCR (qPCR) is a common application in biological and biomedical laboratories for detecting and quantifying specific nucleic acid templates in complex sample mixtures. Generally, qPCR is carried out in one of two ways:

  1. qPCR is carried out in the presence of specific molecular dyes that bind to amplified DNA molecules, whereupon they fluoresce. The fluorescence of these dye molecules is then detected and optically measured in a qPCR machine.
  2. qPCR is performed using nucleic acid probes, which are complementary to the nucleic acid template of interest and emit fluorescence by binding to amplified DNA molecules, or by being degraded by DNA polymerases.

Learn how Liquidator 96 is used for both dye- and probe-based qPCR protocols while enhancing qPCR setup.

Thank you for visiting www.mt.com. We have tried to optimize your experience while on the site, but we noticed that you are using an older version of a web browser. We would like to let you know that some features on the site may not be available or may not work as nicely as they would on a newer browser version. If you would like to take full advantage of the site, please update your web browser to help improve your experience while browsing www.mt.com.