Optical Purity, Ibuprofen - METTLER TOLEDO

Optical Purity, Ibuprofen

SampleIbuprofen

Ibuprofen, racemate (USP reference) and (+) enantiomer

 

Application

Active ingredient

 

Conditions

Measuring cellDSC820

Pan: Aluminum 40 µl, hermetically sealed

Sample preparation: As received, the mixtures are dissolved in a little methanol and left to crystallize slowly for several days at 6 °C

DSC measurement: Heating from 30 °C to 110 °C at 5 K/min

Atmosphere: Nitrogen, 50 cm3/min

 

Interpretation

Isomers are substances with the same chemical composition but different structure. Enantiomers are isomers which show optical activity. If the (+) and (-) enantiomers are present in the same ratio in a crystal, then it is known as a racemate. Racemates and pure enantiomers have different crystal structures, different solubilities and melting points and can therefore be distinguished from each other by DSC. The (+) enantiomer is, in this case, the pharmacologically active form. Compared with the racemate, it has a greater solubility and a lower melting point. This can have consequences in the formulation of pharmaceutical preparations, if for example, the racemate and enantiomer should be able to be replaced by one another.

 

Evaluation

The onset temperatures and heats of fusion of the peaks are determined. The onset temperatures are required for constructing the phase diagram.

SampleOnset, °CΔH, J/gOnset, °CΔH, J/g
Racemate--74.4121.8
(+) Enantiomer50.486.6--
Mixtures
95% enantiomer / 5% racemate47.581.7--
90% enantiomer / 10% racemate47.677.4--
75% enantiomer / 25% racemate43.635.763.739.4

Note: 25% racemate corresponds to 12.5% (-) enantiomer

 

 

The diagram shows the onset temperature as a function of the enantiomer concentration (weight %).

The phase diagram (liquidus curve) shows the onset temperatures as a function of the (-) enantiomer concentration. A concentration of 0% means 100% (+) enantiomer, 50% corresponds to the racemate, and 100% is pure (-) enantiomer. The right section of the diagram (concentration 50% bis 100%) was obtained by forming the mirror image of the left part.

 

Conclusion

DSC can distinguish between racemates and the pure enantiomers. A contamination of the (+) enantiomer with racemate can be detected. This is observed as a broadening of the melting peak and a depression of the melting point.

DSC can not, however, distinguish between the pure (+) and (-) enantiomers because they have the same melting points. One can, however, add the (+) form to the unknown enantiomer: an unchanged melting peak shows that the unknown substance is also present in the (+) form.

 

Optical Purity, Ibuprofen | Thermal Analysis Application No. HB825 | Application published in METTLER TOLEDO TA Application Handbook Pharmaceuticals