Accurate, dependable pH measurement in single -use bioreactor bags plays a critical role in batch productivity and yield. The Mettler Toledo In-Sus 310 single-use pH sensor is designed to meet the challenges of the modern bioprocessing environment. This fully integrated gamma irradiation sterilizable pH sensor is as accurate and reliable as our reusable glass sensors. The Insus 310 is protected during transport and pre-batch preparation by round edged protective guards to prevent the three glass elements from breaking. Its robust design ensures complete sensor and bag integrity. The sensor also prevents the release of electrolyte and particulate into the bag during dry storage with a special media soluble, biocompatible protective coating. Insus 310 provides a reliable measurement signal for at least six weeks. This long -term measurement stability enables use of the sensor in intensified bioprocessing, semi-continuous and perfusion processes, ensuring batch-to-batch consistency. The sensor has an industry-leading 30 -month shelf life. This allows enhanced inventory control at bag manufacturers and end -user sites. The improved availability of single-use bioprocess equipment allows quick adaptation to increased market demands. Thanks to the highest bag and sensor integrity, a long, accurate measurement lifetime, and improved bag availability, operators can focus on running batches without worrying about sensor issues. The Insus 310 pH sensor is available in disposable bags for cell culture, fermentation, and mixing applications from leading producers of bioprocess equipment in single-use format. When accuracy and reliability matter, request Mettler Toledo InSus 310 single-use pH sensors to be installed in your bioreactor bags. Talk to your bag supplier today. To find out more, please visit www.mt .com slash InSus.
The deployment of single-use bioreactors (SUBs) is well established in the bioprocessing industry. SUB technology for mammalian cell culture and microbial cells has achieved major breakthroughs in process safety and reliability to comply with the industry’s strict requirements.
This application note explains the differences in operation of pH and DO single-use and reusable sensors when employed in single-use bioreactors.
Regardless of whether reusable or single-use pH and DO sensors are selected for your SUBs, METTLER TOLEDO can offer highly reliable sensors of both types. Single-use InSUS 310 sensors for pH, and InSUS 607 sensors for DO measurement are supplied as integrated components in SUB assemblies from leading single-use stirred-tank bioreactor manufacturers.
The METTLER TOLEDO InSUS sensor series for single-use technology bioprocesses covers:
- Potentiometric pH sensors with a measurement range from pH 3–10
- Optical dissolved O2 sensors based on fluorescence quenching measurement principle
The output signals of InSUS sensors are identical to most of the common reusable sensors. Consequently, seamless integration into an existing transmitter or biocontroller process environment is straightforward. Inventory control of bags, both at bag manufacturer and end-user sites, is enhanced thanks to a pH sensor shelf life of up to 30 months. The sensors’ long term measurement stability allows for reliable use, especially in intensified bioprocesses.
