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ISO 17025 accredited labs deliver quality service. The requirements vary slightly from country to country, but all focus on a tightly controlled laboratory environment:
Other requirements include published measurement uncertainties, a documented technician proficiency test program and documented processes.
According to the internationally recognized ISO 8655 standard, small volume pipettes like 2µL or even 10µL require a 6 or even 7 digit balance. These balances are extremely sensitive and more often than not fail to work properly in an uncontrolled laboratory environment.
ISO 8655 defines the useful volume range of a pipette as being from 10% of nominal to 100% of nominal. The most commonly used volume settings take after the ISO standard; the nominal volume (100%), 50% of nominal and the lower limit or 10% of nominal volume (ISO 8655-6:2002 section 7.1.1).
Volume settings can sometimes vary depending on the calibration standard applied. For example, certain service providers have adopted 25% for minimum, 75% for medium and 100% for maximum as their volume settings. This unfortunately negates the use of the pipette for volumes settings below 25% of nominal volume.