Water Content Determination of Gelatin with Karl Fischer Titration

Determine Water Content of Gelatin with Karl Fischer Titration

An Automated Method for the External Dissolution of Capsule Shells

This application note gives an automated method for determining the water content of gelatin capsule samples by volumetric Karl Fischer titration using the external dissolution method.

By downloading this application package, you will receive a PDF of the application note as well as the required data for the LabX™ titration software method.

 

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What Is the Importance of Water Content Determination in Gelatin?

The water content in gelatin is crucial to ensure the longevity and efficacy of the enclosed drug. If there is an inappropriate amount of moisture in the gelatin capsules, it can cause damage to the active ingredient, leading to its degradation and reduced effectiveness. Therefore, it is important to minimize the water content in gelatin to prolong the shelf life of the medicine and maintain its potency.

Gelatin is a highly digestible substance produced by partial hydrolysis of collagen extracted from animal tissues and is commonly used as a capsule to contain the active ingredient and filler. In addition to this, gelatin can increase the bioavailability of poorly absorbed or poorly soluble nutrients in the body.

 

How Is Water Content Determined in Gelatin?

Karl Fischer reagent is applied to determine water in gelatin; however, gelatin is only partially dissolved in the reagent leading to incomplete water recovery. A practical alternative is to extract the water by the external dissolution method.

 

Karl Fischer Titration Guide Set
Water Content of Gelatin by Karl Fischer Titration
Water Content of Gelatin by Karl Fischer Titration