{ "timestamp": "2026/10/07 18:43:00", "device_name": "iPad", "device_model": "iPad", "operating_system": "iPadOS 18.6", "app_version": "4.5.41", "app_identifier": "617083206-2026/03/31 16:32:01", "programs": [ { "pipettor_type": "MicroPro-300", "type": "Custom Program", "name": "Magnetic Bead DNA Clean Up", "description": "", "created": "2026/10/07 18:42:46", "last_edited": "2026/10/07 18:42:46", "tip_type": "300ul", "version": 9, "actions": [ { "step_number": 1, "action": "INSTRUCTIONS", "headline": "Magnetic Bead DNA Clean Up", "instructions": "This method is a starting template. Verify and optimize tip position, pipetting speeds, mixing, magnetic separation time, drying time, and transfer volume for selected labware, magnet, samples, and application.\n\nFor easier and more reproducible operation, establish and save tip-height settings (pipetting depth recall) for each labware type before running the method. See manual section 4.6 for detailed instructions.", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 2, "action": "INSTRUCTIONS", "headline": "Mag Bead Clean Up", "instructions": "Bring beads to room temperature and fully resuspend. \nPrepare fresh 70% ethanol.\nTransfer sufficient beads to reservoir or PCR plate for addition to sample.\nAdd sufficient 70% ethanol to reservoir.\nAdd sufficient elution buffer into reservoir.\n", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 3, "action": "INSTRUCTIONS", "headline": "Add beads to sample", "instructions": "", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 4, "action": "ASPIRATE", "amount": "36.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 5, "action": "DISPENSE", "amount": "36.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 6, "action": "MIX", "amount": "42.0ul", "speed": 8, "plate": "", "mixes": 10, "mix_add_blowout_if_zero_volume": 1, "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 7, "action": "INSTRUCTIONS", "headline": "Incubate for DNA binding", "instructions": "Incubate the plate for 5 minutes.\n", "wait_to_run": "YES", "wait_to_run_seconds": 300, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 8, "action": "INSTRUCTIONS", "headline": "Separate Beads", "instructions": "Place sample plate on magnet.\nIncubate for 5 minutes, or until sample is visibly clear.\nRemove supernatant and dispense into waste container.", "wait_to_run": "YES", "wait_to_run_seconds": 300, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 9, "action": "ASPIRATE", "amount": "51.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 10, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "51.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 11, "action": "TIP CHANGE", "tip_change_type": "eject/load", "require_filter_tips_message": "NO", "tip_type": "300ul", "allow_manual_tip_load": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 12, "action": "INSTRUCTIONS", "headline": "Ethanol Wash 1", "instructions": "Keep the sample plate on thr magnetic. Add 200uL ethanol to each well.", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 13, "action": "ASPIRATE", "amount": "200.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 14, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "200.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 15, "action": "INSTRUCTIONS", "headline": "Incubate", "instructions": "Incubate for 30s at room temperature. Keep sample plate on magnetic stand.\nRemove and dispense into waste container.", "wait_to_run": "YES", "wait_to_run_seconds": 30, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 16, "action": "ASPIRATE", "amount": "200.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 17, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "200.0ul", "speed": 5, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 18, "action": "TIP CHANGE", "tip_change_type": "eject/load", "require_filter_tips_message": "NO", "tip_type": "300ul", "allow_manual_tip_load": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 19, "action": "INSTRUCTIONS", "headline": "Ethanol Wash 2", "instructions": "Keep sample plate on magnet. Add 200uL of ethanol to each sample well.", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 20, "action": "ASPIRATE", "amount": "200.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 21, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "200.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 22, "action": "INSTRUCTIONS", "headline": "Incubate", "instructions": "Incubate 30s at room temperature.\nRemove and dispense into waste container.\nKeep sample plate on magnet. ", "wait_to_run": "YES", "wait_to_run_seconds": 30, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 23, "action": "ASPIRATE", "amount": "205.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 24, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "205.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 25, "action": "TIP CHANGE", "tip_change_type": "eject/load", "require_filter_tips_message": "NO", "tip_type": "300ul", "allow_manual_tip_load": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 26, "action": "INSTRUCTIONS", "headline": "Dry Beads", "instructions": "Keep sample plate on magnetic stand.\nAir dry beads for 2 minutes.\nDo not overdry beads, optimization of drying time may be required.", "wait_to_run": "YES", "wait_to_run_seconds": 120, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 27, "action": "INSTRUCTIONS", "headline": "Elution", "instructions": "Remove sample plate from magnet.\nAdd elution buffer to each well and mix.", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 28, "action": "ASPIRATE", "amount": "40.0ul", "speed": 8, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 29, "action": "DISPENSE", "amount": "40.0ul", "speed": 5, "plate": "", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 30, "action": "MIX", "amount": "25.0ul", "speed": 8, "plate": "", "mixes": 10, "mix_add_blowout_if_zero_volume": 1, "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" }, { "step_number": 31, "action": "TIP CHANGE", "tip_change_type": "eject/load", "require_filter_tips_message": "NO", "tip_type": "300ul", "allow_manual_tip_load": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 32, "action": "INSTRUCTIONS", "headline": "Incubate for elution", "instructions": "Incubate at 5 minutes at room temperature. Elution time may be optimized for the sample type and required recovery.", "wait_to_run": "YES", "wait_to_run_seconds": 300, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 33, "action": "INSTRUCTIONS", "headline": "Separate beads", "instructions": "Place sample plate on magnet and incubate for 2 minutes or until samples are visibly clear. Keep the sample on the magnet during the final transfer.", "wait_to_run": "YES", "wait_to_run_seconds": 120, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 34, "action": "INSTRUCTIONS", "headline": "Transfer purified sample", "instructions": "Aspirate the eluate from the sample plate and transfer to destination plate.\n\nThis eulate contains purified sample, do not discard.", "wait_to_run": "NO", "wait_to_run_seconds": 0, "exit_instruction_screen_when_wait_to_run_timer_expires": "NO", "can_go_to_after_purge": "NO", "automatically_run": "NO" }, { "step_number": 35, "action": "ASPIRATE", "amount": "40.0ul", "speed": 5, "plate": "", "can_go_to_after_purge": "NO", "change_liquid_class": "NO", "liquid_class": "", "automatically_run": "NO" }, { "step_number": 36, "action": "DISPENSE WITH BLOWOUT AND BLOWIN", "amount": "40.0ul", "speed": 5, "plate": "", "can_go_to_after_purge": "NO", "blowout_volume": "9.2ul", "automatically_run": "NO" } ] } ] }