During ozone sanitization, it is typical to observe a temporary increase in TOC levels. This spike indicates that ozone is effectively breaking down complex organic contaminants into smaller organic compounds and byproducts. Once the sanitization cycle concludes and the system is flushed, TOC levels typically return to baseline.

Ozone significantly reduces bacterial counts by effectively inactivating bacteria, viruses, and other microorganisms, preventing their replication. Following an ozone sanitization cycle, microbial counts are substantially decreased or even eliminated. This can be clearly seen with a real-time bioburden analyzer that displays microbial counts. Plate counts cannot detect this trend in the water system to the same level as a bioburden analyzer, but the correlation between ozonation and microbial counts is clear.