PureSpeed C18 Tips
Reverse phase adsorption is a technique often used to desalt and concentrate biomolecules such as peptides and proteins. Used extensively for sample preparation, a hydrophobic stationary phase comprising alkylsilane- modified silica particles is used to retain hydrophobic molecules.
Hydrophilic molecules or salts are not retained by the stationary phase so these species are removed from the sample. After washing steps to remove residual hydrophilic molecules and salts, the hydrophobic molecules of interest are eluted (recovered) from the stationary phase by a non-polar solvent. The usually volatile non-polar solvent evaporates from the final sample and the pellet can be dissolved in aqueous buffers.