Sucrose Content - UV Vis Spectroscopy

This spectroscopy application note shows how to determine Sucrose in Apple Juice by using the UV7 Spectrophotometer from METTLER TOLEDO. For more information about this application, proceed to download the method below.

How is Sucrose Content Measured Spectrophotometrically?

Sucrose is a disaccharide composed of glucose and fructose. The sucrose Assay kit is used for the quantitative enzymatic determination of sucrose in food and other materials. The sucrose is hydrolyzed to glucose and fructose by the enzyme invertase.

This glucose and fructose is phosphorylated to Glucose - 6 - phosphate (G6P) by ATP (Adenosine triphosphate) with hexokinase as a catalysator. Afterwards, G6P dehydrogenase (G6PDH) catalyzes the reaction, where nicotinamide adenine dinucleotide (NAD) oxidizes the G6P to 6-phosphogluconate. During this oxidation, NAD is reduced to NADH. Because NADH absorbs at 340 nm. The increase in absorbance is directly proportional to the reduced NADH and to the sucrose concentration.

For more detailed experimental and results information, please download the application note below.