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Espectrofotómetros UV-VIS
Instruments, Accessories, Services, and Software for UV Vis Spectrophotometry
Spectrophotometry is a powerful technique used to measure a sample's light absorption and transmission properties, providing valuable insights into its composition and chemical properties. Whether you're working in life sciences, quality control, environmental science, or any other field where accurate and reliable measurements are critical to success, our spectrophotometric solutions offer performance, ease of use, and scalability, helping you achieve faster, more precise results with every analysis.
FAQ
What is UV Vis spectrophotometry?
Spectrophotometry is the measurement of light absorption by a substance as a function of wavelength. It is commonly used in analytical chemistry, biochemistry, and molecular biology.
What are the applications of UV Vis spectrophotometry?
UV Vis spectrophotometry can be used to determine the concentration of a solution, to identify and quantify the components of a mixture, and to study the kinetics of a reaction.
Find our more in the UV Vis Application Library
What is the difference between spectrophotometry and spectroscopy?
Spectroscopy is a broader term that refers to the study of the interaction between electromagnetic radiation and matter. Spectrophotometry is a specific type of spectroscopy that measures the absorption of light by a substance.
Learn more about the theory of UV Vis spectrophotometry in the Easy Spectrophotometry Guide or the Spectrophotometry Applications and Fundamentals Guide
How does UV Vis spectrophotometry work?
A UV Vis spectrophotometer measures the amount of light absorbed by a sample at a specific wavelength. The amount of light absorbed is directly proportional to the concentration of the substance in the sample.
What are the advantages of using UV Vis spectrophotometry?
UV Vis spectrophotometry is a fast, accurate, and non-destructive method of analyzing samples. It can be used for a wide range of substances and is widely accepted as a standard analytical technique in many industries.
How is UV Vis spectrophotometry used in the pharmaceutical industry?
UV Vis spectrophotometry is used in the pharmaceutical industry to analyze the purity, identity, and strength of drugs. It can also be used to monitor the degradation of drugs over time.
To learn more about how spectrophotometry can ensure therapeutic quality, read our customer story
What is a blank in UV Vis spectrophotometry?
A blank is a sample that contains all the reagents except the substance being tested. It is used to calibrate the spectrophotometer and to correct for any background absorption or interference.
Can UV Vis spectrophotometry be used to analyze biological samples?
Yes, UV Vis spectrophotometry can be used to analyze biological samples such as proteins, nucleic acids, and enzymes. It can also be used to quantify the amount of a particular compound in a biological sample.
Learn more in the Life Science Toolbox for UV Vis Spectrophotometry
How can I ensure the accuracy of my spectrophotometry measurements?
To ensure accuracy, it is important to properly calibrate the spectrophotometer, use appropriate reagents and standards, and follow a standardized protocol for sample preparation and measurement. It is also important to validate your results by comparing them to other methods of analysis.
Find out more about the METTLER TOLEDO service offerings for UV Vis calibration
¿Qué métodos de ciencias de la vida están disponibles en un espectrofotómetro de METTLER TOLEDO?
- Los espectrofotómetros UV5Nano y UV5Bio de METTLER TOLEDO ofrecen una amplia variedad de métodos de ciencias de la vida como:
- Análisis cualitativo de ADN, ARN y proteínas
- Análisis cuantitativo de ADN, ARN y proteínas
- BCA, Bradford, Lowry y otros ensayos proteicos clorométricos
- Colorantes configurados previamente y la opción de añadir colorantes personalizados
- Calculadora de oligonucleótidos para la determinación de la concentración de oligonucleótidos de ADN y ARN
- OD600 para las células vivas
- Análisis cualitativo de ADN, ARN y proteínas
- De forma adicional, los espectrofotómetros UV5Bio y UV7 ofrecen métodos cinéticos para desarrollar cinética enzimática
- Para más información sobre las aplicaciones de ciencias de la vida de los espectrofotómetros de METTLER TOLEDO, descárguese el conjunto de herramientas de ciencias de la vida.
¿Qué escalas de color y números proporcionan los colorímetros de METTLER TOLEDO?
Los espectrofotómetros de METTLER TOLEDO incluyen varias escalas de color preinstaladas como APHA, Gardner, Saybolt, CIELAB, EBC y ASBC, lo que hace que sea una solución ideal para realizar mediciones de color de líquidos transparentes.
El espacio de color APHA (sinónimo de Pt-Co y Hazen), así como la escala de colores Gardner, cuantifica la amarillez de las sustancias casi transparentes y, por lo tanto, sirve para determinar la pureza y la calidad, o el grado de degradación de una sustancia.
CIELAB expresa el color con tres valores: L* para la luminosidad, a* para verde a rojo y b* para azul a amarillo. Esta escala de colores se emplea para asegurar un color uniforme de los líquidos, por ejemplo, de los tintes, sabores, etc.
La escala de colores Saybolt se usa para clasificar los productos petrolíferos de color claro. El color Saybolt es un indicador de la calidad o el grado de contaminación de productos como la gasolina, el combustible para aviones, etc.
Las escalas de colores EBC y ASBC se usan para determinar el color de la cerveza, lo que distingue los tipos de cerveza.